Rv1347c Lysine N-acyltransferase mbtK (EC 2.3.1.-) (N-acyltransferase mbtK) (Mycobactin synthetase protein K)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1347c mbtK Lysine N-acyltransferase mbtK (EC 2.3.1.-) (N-acyltransferase mbtK) (Mycobactin synthetase protein... CDS 1511973 1512605 - 633 210 FALSE

Rv1347c (Lysine N-acyltransferase mbtK (EC 2.3.1.-) (N-acyltransferase mbtK) (Mycobactin synthetase protein K)) is predicted to be co-regulated in modules bicluster_0343 with residual 0.46 and bicluster_0502 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 390.00 and 13,000.00 for bicluster_0343 and 0.00 and 1.20 for bicluster_0502 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0343
e.value: 
390
Motif Bicluster: 
e.value: 
13000
Motif Bicluster: 
0.46
bicluster_0502
e.value: 
2e-16
Motif Bicluster: 
e.value: 
1.2
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
Lysine N-acyltransferase mbtK
Operon # Operon
906
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608487 NP_215863.1 Run
GO:0008415

NA

NA

Details: 
NA
GO Category: 
NA
6
Total items in this category:  
GO:0016291

NA

NA

Details: 
NA
GO Category: 
NA
2
Total items in this category:  
GO:0016410

N-acyltransferase activity

N-acyltransferase activity

Details: 
Catalysis of the transfer of an acyl group to a nitrogen atom on the acceptor molecule.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0019290

siderophore biosynthetic process

siderophore biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of siderophores, low molecular weight Fe(III)-chelating substances made by aerobic or facultatively anaerobic bacteria, especially when growing under iron deficient conditions. The complexes of Fe(3+)-siderophores have very high stability constants and are taken up by specific transport systems by microorganisms; the subsequent release of iron requires enzymatic action.
GO Category: 
biological_process
6
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0034069

aminoglycoside N-acetyltransferase activity

aminoglycoside N-acetyltransferase activity

Details: 
Catalysis of the reaction: acetyl-CoA + aminoglycoside = CoA + N-acetylaminoglycoside.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.420000 10.51

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: