Rv1350 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1350 fabG2 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) CDS 1517491 1518234 + 744 247 FALSE

Rv1350 (3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)) is predicted to be co-regulated in modules bicluster_0419 with residual 0.50 and bicluster_0580 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 10.00 for bicluster_0419 and 0.00 and 0.19 for bicluster_0580 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0419
e.value: 
0.024
Motif Bicluster: 
e.value: 
10
Motif Bicluster: 
0.50
bicluster_0580
e.value: 
0.0000012
Motif Bicluster: 
e.value: 
0.19
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Uncharacterized oxidoreductase Rv1350_MT1393 3-ketoacyl-(acyl-carrier-protein) reductase
Operon # Operon
908 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3-oxoacyl-[acyl-carrier-protein] reductase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Biosynthesis of unsaturated fatty acids

10
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608490 NP_215866.1 Run
GO:0004316

3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

Details: 
Catalysis of the reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.150000 0.29

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: