Rv1406 Methionyl-tRNA formyltransferase (EC 2.1.2.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1406 fmt Methionyl-tRNA formyltransferase (EC 2.1.2.9) CDS 1582166 1583104 + 939 312 FALSE

Rv1406 (Methionyl-tRNA formyltransferase (EC 2.1.2.9)) is predicted to be co-regulated in modules bicluster_0431 with residual 0.49 and bicluster_0538 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.20 and 70.00 for bicluster_0431 and 21.00 and 880.00 for bicluster_0538 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Methionyl-tRNA formyltransferase methionyl-tRNA formyltransferase
Operon # Operon
944 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

One carbon pool by folate

13
Total items in this category:  

KEGG

Aminoacyl-tRNA biosynthesis

69
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608544 NP_215922.1 Run
GO:0004479

methionyl-tRNA formyltransferase activity

methionyl-tRNA formyltransferase activity

Details: 
Catalysis of the reaction: 10-formyltetrahydrofolate + L-methionyl-tRNA + H2O = tetrahydrofolate + N-formylmethionyl-tRNA.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: