Rv1451 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1451 ctaB Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB CDS 1635029 1635955 + 927 308 FALSE

Rv1451 (Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB) is predicted to be co-regulated in modules bicluster_0074 with residual 0.41 and bicluster_0215 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 450.00 and 850.00 for bicluster_0074 and 13.00 and 140.00 for bicluster_0215 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Protoheme IX farnesyltransferase protoheme IX farnesyltransferase
Operon # Operon
968
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Porphyrin and chlorophyll metabolism

31
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608589 NP_215967.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: