Rv1471 Thioredoxin TrxB1

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1471 trxB1 Thioredoxin TrxB1 CDS 1659370 1659741 + 372 123 FALSE

Rv1471 (Thioredoxin TrxB1) is predicted to be co-regulated in modules bicluster_0066 with residual 0.56 and bicluster_0546 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 47.00 and 3,300.00 for bicluster_0066 and 0.00 and 0.21 for bicluster_0546 respectively.

These modules are enriched for following go terms: glycerol ether metabolic process, ether metabolic process, protein disulfide oxidoreductase activit..., disulfide oxidoreductase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
RNA polymerase sigma-54 factor RpoN
Induced 9 2.94 3.57e-16 CDS
transcriptional regulator, ArsR family
No 36 0.57 0.0398673 CDS
RNA polymerase sigma-70 factor
No 13 -0.3 0.819451 CDS
Motif 1 Motif 2 Residual
bicluster_0066
e.value: 
47
Motif Bicluster: 
e.value: 
3300
Motif Bicluster: 
0.56
bicluster_0546
e.value: 
0.0017
Motif Bicluster: 
e.value: 
0.21
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
PROBABLE THIOREDOXIN TRXB1 thioredoxin TRXB1
Operon # Operon
980 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116870 YP_177815.1 Run
GO:0015035

protein disulfide oxidoreductase activity

protein disulfide oxidoreductase activity

Details: 
Catalysis of the reaction: a protein with reduced sulfide groups = a protein with oxidized disulfide bonds.
GO Category: 
molecular_function
7
Total items in this category:  
GO:0045454

cell redox homeostasis

cell redox homeostasis

Details: 
Any process that maintains the redox environment of a cell or compartment within a cell.
GO Category: 
biological_process
9
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.220000 0.48

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: