Rv1474c Transcriptional regulator, TetR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1474c Transcriptional regulator, TetR family CDS 1662641 1663204 - 564 187 FALSE

Rv1474c (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0516 with residual 0.52 and bicluster_0577 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,900.00 and 10,000.00 for bicluster_0516 and 150.00 and 4,700.00 for bicluster_0577 respectively.

These modules are enriched for following go terms: anion transport, external encapsulating structure, cell periphery, transmembrane transporter activity, transporter activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN transcriptional regulatory protein
Operon # Operon
982 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608612 NP_215990.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.460000 0.85

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00000430546
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 11.39 U
D3I 3 3 3.83 11.25 I
D3U 3 3 3.83 11.02 U
D5I 9 5 6.00 14.31 I
D5U 17 5 6.00 14.22 U
D7I 18 7 8.14 11.87 I
D7U 19 7 8.14 10.92 U
D14I 4 14 15.63 9.86 I
D14U 4 14 15.63 9.46 U
D17I 3 17 19.15 8.66 I
D17U 3 17 19.15 8.79 U
D21I 4 21 23.23 8.81 I
D21U 4 21 23.23 9.10 U
D24I 3 24 26.60 7.91 I
D24U 3 24 26.60 8.35 U
D28I 4 28 30.61 8.30 I
D28U 4 28 30.61 9.04 U