Rv1533 Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1533 Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9) CDS 1733610 1734737 + 1 128 375 FALSE

Rv1533 (Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)) is predicted to be co-regulated in modules bicluster_0138 with residual 0.67 and bicluster_0580 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.42 for bicluster_0138 and 0.00 and 0.19 for bicluster_0580 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator TrcR
No -32 -0.03 0.968459 Primary.TSS
Transcriptional regulator, PadR family
No -24 -0.13 0.687699 Primary.TSS
Histone protein Lsr2
No -20 0.18 0.498703 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0138
e.value: 
0.00000018
Motif Bicluster: 
e.value: 
0.42
Motif Bicluster: 
0.67
bicluster_0580
e.value: 
0.0000012
Motif Bicluster: 
e.value: 
0.19
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
1020 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Enoyl-[acyl-carrier-protein] reductase (NADH) Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608671 NP_216049.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.580000 1.19

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: