Rv1542c Cyanoglobin; Hemoglobin-like protein HbN

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1542c glbN Cyanoglobin; Hemoglobin-like protein HbN CDS 1744426 1744836 - 411 136 FALSE

Rv1542c (Cyanoglobin; Hemoglobin-like protein HbN) is predicted to be co-regulated in modules bicluster_0069 with residual 0.54 and bicluster_0550 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0069 and 0.23 and 6.90 for bicluster_0550 respectively.

These modules are enriched for following go terms: cyclohydrolase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Rv1542c hemoglobin glbN
Operon # Operon
1027
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608680 NP_216058.1 Run
GO:0019825

oxygen binding

oxygen binding

Details: 
Interacting selectively and non-covalently with oxygen (O2).
GO Category: 
molecular_function
4
Total items in this category:  
GO:0020037

heme binding

heme binding

Details: 
Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO Category: 
molecular_function
9
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0046210

nitric oxide catabolic process

nitric oxide catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of nitric oxide, nitrogen monoxide (NO), a colorless gas only slightly soluble in water.
GO Category: 
biological_process
1
Total items in this category:  
GO:0051410

detoxification of nitrogen compound

detoxification of nitrogen compound

Details: 
Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.
GO Category: 
biological_process
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.650000 1.40

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: