Rv1549 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1549 fadD11.1 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) CDS 1753510 1754037 + 528 175 FALSE

Rv1549 (Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)) is predicted to be co-regulated in modules bicluster_0429 with residual 0.52 and bicluster_0434 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.40 and 2,100.00 for bicluster_0429 and 0.00 and 0.00 for bicluster_0434 respectively.

These modules are enriched for following go terms: transferase activity, transferring one-c... cellular macromolecule catabolic process, cellular protein metabolic process, coenzyme biosynthetic process, cofactor biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.179 1753606 1753510
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0429
e.value: 
1.4
Motif Bicluster: 
e.value: 
2100
Motif Bicluster: 
0.52
bicluster_0434
e.value: 
0.0000018
Motif Bicluster: 
e.value: 
0.000029
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1549_MT15991 fatty-acid-CoA ligase
Operon # Operon
1032 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Long-chain-fatty-acid--CoA ligase Fatty acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Caprolactam degradation

27
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116883 YP_177818.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.410000 0.72

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: