Rv1570 Dethiobiotin synthetase (EC 6.3.3.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1570 bioD Dethiobiotin synthetase (EC 6.3.3.3) CDS 1777859 1778539 + 681 226 FALSE

Rv1570 (Dethiobiotin synthetase (EC 6.3.3.3)) is predicted to be co-regulated in modules bicluster_0041 with residual 0.83 and bicluster_0217 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 48.00 for bicluster_0041 and 0.00 and 0.09 for bicluster_0217 respectively.

These modules are enriched for following go terms: biosynthetic process, cellular amide metabolic process, monocarboxylic acid metabolic process, amide binding, amino acid binding, vitamin binding vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce..., cofactor metabolic process, carboxylic acid biosynthetic process, organic acid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Dethiobiotin synthetase dithiobiotin synthetase
Operon # Operon
1039 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Dethiobiotin synthase Biotin metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biotin metabolism

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608708 NP_216086.1 Run
GO:0004141

dethiobiotin synthase activity

dethiobiotin synthase activity

Details: 
Catalysis of the reaction: 7,8-diaminononanoate + ATP + CO(2) = ADP + dethiobiotin + 4 H(+) + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: