Rv1589 Biotin synthase (EC 2.8.1.6)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1589 bioB Biotin synthase (EC 2.8.1.6) CDS 1790284 1791333 + 1 050 349 FALSE

Rv1589 (Biotin synthase (EC 2.8.1.6)) is predicted to be co-regulated in modules bicluster_0065 with residual 0.72 and bicluster_0491 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 7.10 and 1,500.00 for bicluster_0065 and 0.01 and 4,200.00 for bicluster_0491 respectively.

These modules are enriched for following go terms: UDP-glycosyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.015 36 1790200 1790236 1790284
Product (LegacyBRC) Product (RefSeq)
Rv1589 biotin synthase
Operon # Operon
1048 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Biotin synthase Biotin metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biotin metabolism

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608727 NP_216105.1 Run
GO:0004076

biotin synthase activity

biotin synthase activity

Details: 
Catalysis of the reaction: 2 S-adenosyl-L-methionine + dethiobiotin + S(2-) = 2 5'-deoxyadenosine + 2 L-methionine + biotin + H(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: