Rv1618 Acyl-CoA thioesterase II (EC 3.1.2.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1618 tesB1 Acyl-CoA thioesterase II (EC 3.1.2.-) CDS 1817615 1818517 + 903 300 FALSE

Rv1618 (Acyl-CoA thioesterase II (EC 3.1.2.-)) is predicted to be co-regulated in modules bicluster_0082 with residual 0.54 and bicluster_0407 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,300.00 and 10,000.00 for bicluster_0082 and 0.10 and 1.70 for bicluster_0407 respectively.

These modules are enriched for following go terms: GTPase activity, GTP binding, guanyl nucleotide binding, guanyl ribonucleotide binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:39
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18426 MT1654 2251
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, AsnC family
No -62 -0.02 0.983009 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0082
e.value: 
1300
Motif Bicluster: 
e.value: 
10000
Motif Bicluster: 
0.54
bicluster_0407
e.value: 
0.096
Motif Bicluster: 
e.value: 
1.7
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
Probable acyl-CoA thioesterase II tesB1 acyl-CoA thioesterase II TesB1
Operon # Operon
1061 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biosynthesis of unsaturated fatty acids

10
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608756 NP_216134.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.110000 0.94

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: