Rv1632c

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1632c CDS 1836387 1836830 - 444 147 FALSE

Rv1632c () is predicted to be co-regulated in modules bicluster_0438 with residual 0.51 and bicluster_0554 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.50 and 3,200.00 for bicluster_0438 and 8.40 and 31.00 for bicluster_0554 respectively.

These modules are enriched for following go terms: porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet..., cofactor biosynthetic process, sulfur amino acid biosynthetic process, sulfur amino acid metabolic process, cofactor metabolic process cellular amide metabolic process, amide biosynthetic process.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:39
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14759 MT1668 236
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Lactate-responsive regulator LldR in Enterobacteria, GntR family
Repressed -3 -1.41 0.000000000007 Primary.TSS
No -127 0.24 0.999609 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0438
e.value: 
2.5
Motif Bicluster: 
e.value: 
3200
Motif Bicluster: 
0.51
bicluster_0554
e.value: 
8.4
Motif Bicluster: 
e.value: 
31
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
1070 Rv1632c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608770 NP_216148.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.090000 0.74

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.