Rv1652 N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1652 argC N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) CDS 1865576 1866634 + 1 059 352 FALSE

Rv1652 (N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38)) is predicted to be co-regulated in modules bicluster_0271 with residual 0.56 and bicluster_0521 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.03 for bicluster_0271 and 0.00 and 0.01 for bicluster_0521 respectively.

These modules are enriched for following go terms: carboxylic acid biosynthetic process, organic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.19 15 1865591 1865591 1865576
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 5 of 5
ChipSeq TF Differential Expression Distance Expression pvalue Type
Arginine pathway regulatory protein ArgR, repressor of arg regulon
Induced -17 3.02 1.43e-19 CDS
Two component transcriptional regulatory protein DevR
No -138 0.47 0.0398551 CDS
transcriptional regulator, ArsR family
No -12 0.4 0.11771 CDS
transcriptional regulator, ArsR family
No -27 0.4 0.11771 Internal.TSS
Arginine pathway regulatory protein ArgR, repressor of arg regulon
Induced -32 3.02 1.43e-19 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0271
e.value: 
0.013
Motif Bicluster: 
e.value: 
0.027
Motif Bicluster: 
0.56
bicluster_0521
e.value: 
0.0011
Motif Bicluster: 
e.value: 
0.0096
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
N-acetyl-gamma-glutamyl-phosphate reductase N-acetyl-gamma-glutamyl-phosphate reductase
Operon # Operon
1087 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

N-acetyl-gamma-glutamyl-phosphate reductase Arginine and proline metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Lysine biosynthesis

15
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608790 NP_216168.1 Run
GO:0003942

N-acetyl-gamma-glutamyl-phosphate reductase activity

N-acetyl-gamma-glutamyl-phosphate reductase activity

Details: 
Catalysis of the reaction: N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: