Rv1665 Chalcone synthase (EC 2.3.1.74)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1665 pks11 Chalcone synthase (EC 2.3.1.74) CDS 1891226 1892287 + 1 062 353 FALSE

Rv1665 (Chalcone synthase (EC 2.3.1.74)) is predicted to be co-regulated in modules bicluster_0041 with residual 0.83 and bicluster_0109 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 48.00 for bicluster_0041 and 1.00 and 8.40 for bicluster_0109 respectively.

These modules are enriched for following go terms: biosynthetic process, cellular amide metabolic process, monocarboxylic acid metabolic process, amide binding, amino acid binding, vitamin binding amide binding, amino acid binding, vitamin binding, carboxylic acid binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:42
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18410 MT1705 2213
Product (LegacyBRC) Product (RefSeq)
Possible chalcone synthase pks11 chalcone synthase
Operon # Operon
1091
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Naringenin-chalcone synthase Flavonoid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608803 NP_216181.1 Run
GO:0008610

lipid biosynthetic process

lipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
13
Total items in this category:  
GO:0009715

chalcone biosynthetic process

chalcone biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of chalcone, phenyl steryl ketone or its hydroxylated derivatives.
GO Category: 
biological_process
2
Total items in this category:  
GO:0071766

Actinobacterium-type cell wall biogenesis

Actinobacterium-type cell wall biogenesis

Details: 
A cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a cell wall of the type found in Actinobacteria. The cell wall is the rigid or semi-rigid envelope lying outside the cell membrane. Actinobacterial cell walls contain characteristic mycolic acids, of which some are covalently linked to the cell wall peptidoglycan and others accumulate at the cell surface.
GO Category: 
biological_process
19
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.470000 3.33

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: