Rv1695 NAD kinase (EC 2.7.1.23)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1695 ppnK NAD kinase (EC 2.7.1.23) CDS 1918746 1919669 + 924 307 FALSE

Rv1695 (NAD kinase (EC 2.7.1.23)) is predicted to be co-regulated in modules bicluster_0034 with residual 0.54 and bicluster_0112 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 57.00 and 260.00 for bicluster_0034 and 0.70 and 3.00 for bicluster_0112 respectively.

These modules are enriched for following go terms: phosphotransferase activity, for other s... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0034
e.value: 
57
Motif Bicluster: 
e.value: 
260
Motif Bicluster: 
0.54
bicluster_0112
e.value: 
0.7
Motif Bicluster: 
e.value: 
3
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
Rv1695 inorganic polyphosphate/ATP-NAD kinase
Operon # Operon
1104 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NAD(+) kinase Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608833 NP_216211.1 Run
GO:0003951

NAD+ kinase activity

NAD+ kinase activity

Details: 
Catalysis of the reaction: ATP + NAD(+) = ADP + 2 H(+) + NADP(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0003951

NAD+ kinase activity

NAD+ kinase activity

Details: 
Catalysis of the reaction: ATP + NAD(+) = ADP + 2 H(+) + NADP(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0006741

NADP biosynthetic process

NADP biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; biosynthesis may be of either the oxidized form, NADP, or the reduced form, NADPH.
GO Category: 
biological_process
1
Total items in this category:  
GO:0006797

polyphosphate metabolic process

polyphosphate metabolic process

Details: 
The chemical reactions and pathways involving a polyphosphate, the anion or salt of polyphosphoric acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: