Rv1703c Probable catechol-o-methyltransferase (EC 2.1.1.6)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1703c Probable catechol-o-methyltransferase (EC 2.1.1.6) CDS 1929131 1929721 - 591 196 FALSE

Rv1703c (Probable catechol-o-methyltransferase (EC 2.1.1.6)) is predicted to be co-regulated in modules bicluster_0097 with residual 0.50 and bicluster_0408 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.37 for bicluster_0097 and 0.01 and 0.62 for bicluster_0408 respectively.

These modules are enriched for following go terms: monovalent inorganic cation transmembran..., inorganic cation transmembrane transport... fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.465 483 1929238 -594 49 1929881 1929832 1929721
Last update: 10/16/2017 - 11:42
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15725 MT1743 1623
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0097
e.value: 
0.0000086
Motif Bicluster: 
e.value: 
0.37
Motif Bicluster: 
0.50
bicluster_0408
e.value: 
0.011
Motif Bicluster: 
e.value: 
0.62
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
Probable catechol-o-methyltransferase catechol-o-methyltransferase
Operon # Operon
1108
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608841 NP_216219.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.490000 1.80

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: