Rv1704c D-serine/D-alanine/glycine transporter

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1704c cycA D-serine/D-alanine/glycine transporter CDS 1929786 1931456 - 1 671 556 FALSE

Rv1704c (D-serine/D-alanine/glycine transporter) is predicted to be co-regulated in modules bicluster_0290 with residual 0.50 and bicluster_0320 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 58.00 and 8,200.00 for bicluster_0290 and 12.00 and 350.00 for bicluster_0320 respectively.

These modules are enriched for following go terms: glutamine family amino acid biosynthetic..., glutamine family amino acid metabolic pr....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:42
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14719 MT1744 1253
Product (LegacyBRC) Product (RefSeq)
PROBABLE D-SERINE_ALANINE_GLYCINE TRANSPORTER PROTEIN CYCA D-serine/alanine/glycine transporter protein CycA
Operon # Operon
1109
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608842 NP_216220.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.370000 1.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: