Rv1715 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1715 fadB3 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) CDS 1942659 1943573 + 915 304 FALSE

Rv1715 (3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157)) is predicted to be co-regulated in modules bicluster_0427 with residual 0.47 and bicluster_0584 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 4.60 for bicluster_0427 and 0.30 and 380.00 for bicluster_0584 respectively.

These modules are enriched for following go terms: galactose metabolic process, oxidoreductase activity, acting on the C... nucleobase-containing compound biosynthe..., small molecule metabolic process, cellular nitrogen compound biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE 3-HYDROXYBUTYRYL-CoA DEHYDROGENASE FADB3 [BETA-HYDROXYBUTYRYL-CoA DEHYDROGENASE] [BHBD] 3-hydroxybutyryl-CoA dehydrogenase FADB3
Operon # Operon
1114 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116901 YP_177829.1 Run
GO:0008691

3-hydroxybutyryl-CoA dehydrogenase activity

3-hydroxybutyryl-CoA dehydrogenase activity

Details: 
Catalysis of the reaction: (S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.080000 0.67

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: