Rv1743 Serine/threonine-protein kinase PknE (EC 2.7.11.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1743 pknE Serine/threonine-protein kinase PknE (EC 2.7.11.1) CDS 1969004 1970704 + 1 701 566 FALSE

Rv1743 (Serine/threonine-protein kinase PknE (EC 2.7.11.1)) is predicted to be co-regulated in modules bicluster_0122 with residual 0.57 and bicluster_0298 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.10 and 6.50 for bicluster_0122 and 0.00 and 0.69 for bicluster_0298 respectively.

These modules are enriched for following go terms: cellular protein modification process, protein modification process, phosphorylation, protein kinase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -127 -0.19 0.999609 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0122
e.value: 
1.1
Motif Bicluster: 
e.value: 
6.5
Motif Bicluster: 
0.57
bicluster_0298
e.value: 
0.0000035
Motif Bicluster: 
e.value: 
0.69
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Serine_threonine-protein kinase pknE transmembrane serine/threonine-protein kinase E
Operon # Operon
1131
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608881 NP_216259.1 Run
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0004674

protein serine/threonine kinase activity

protein serine/threonine kinase activity

Details: 
Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0043085

positive regulation of catalytic activity

positive regulation of catalytic activity

Details: 
Any process that activates or increases the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043086

negative regulation of catalytic activity

negative regulation of catalytic activity

Details: 
Any process that stops or reduces the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
GO:0051409

response to nitrosative stress

response to nitrosative stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nitrosative stress stimulus. Nitrosative stress is a state often resulting from exposure to high levels of nitric oxide (NO) or the highly reactive oxidant peroxynitrite, which is produced following interaction of NO with superoxide anions.
GO Category: 
biological_process
14
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.410000 1.03

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: