Rv1746 Probable serine/threonine-protein kinase pknF (EC 2.7.11.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1746 pknF Probable serine/threonine-protein kinase pknF (EC 2.7.11.1) CDS 1972138 1973568 + 1 431 476 FALSE

Rv1746 (Probable serine/threonine-protein kinase pknF (EC 2.7.11.1)) is predicted to be co-regulated in modules bicluster_0055 with residual 0.58 and bicluster_0127 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.58 and 9.50 for bicluster_0055 and 0.00 and 0.02 for bicluster_0127 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Serine_threonine-protein kinase pknF anchored-membrane serine/threonine-protein kinase PKNF (protein kinase F) (STPK F)
Operon # Operon
1133
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608884 NP_216262.1 Run
GO:0000917

barrier septum assembly

barrier septum assembly

Details: 
The assembly and arrangement of a septum that spans the plasma membrane interface between progeny cells following cytokinesis. The progeny cells that form a barrier septum are not able to exchange intracellular material.
GO Category: 
biological_process
2
Total items in this category:  
GO:0001558

regulation of cell growth

regulation of cell growth

Details: 
Any process that modulates the frequency, rate, extent or direction of cell growth.
GO Category: 
biological_process
1
Total items in this category:  
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0004674

protein serine/threonine kinase activity

protein serine/threonine kinase activity

Details: 
Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
GO:0010827

regulation of glucose transport

regulation of glucose transport

Details: 
Any process that modulates the frequency, rate or extent of glucose transport. Glucose transport is the directed movement of the hexose monosaccharide glucose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
1
Total items in this category:  
GO:0043085

positive regulation of catalytic activity

positive regulation of catalytic activity

Details: 
Any process that activates or increases the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043086

negative regulation of catalytic activity

negative regulation of catalytic activity

Details: 
Any process that stops or reduces the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.050000 1.07

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: