Rv1821 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1821 secA2 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) CDS 2066457 2068883 + 2 427 808 FALSE

Rv1821 (Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)) is predicted to be co-regulated in modules bicluster_0056 with residual 0.52 and bicluster_0097 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.11 and 16,000.00 for bicluster_0056 and 0.00 and 0.37 for bicluster_0097 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on a sul..., helicase activity, oxidoreductase activity, acting on a sul... monovalent inorganic cation transmembran..., inorganic cation transmembrane transport....

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-1.311 90 2066547 2066547 2066457
Product (LegacyBRC) Product (RefSeq)
Protein translocase subunit secA 2 preprotein translocase subunit SecA
Operon # Operon
1197 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Protein export

16
Total items in this category:  

KEGG

Bacterial secretion system

14
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608958 NP_216337.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0003676

nucleic acid binding

nucleic acid binding

Details: 
Interacting selectively and non-covalently with any nucleic acid.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0017038

protein import

protein import

Details: 
The directed movement of proteins into a cell or organelle.
GO Category: 
biological_process
2
Total items in this category:  
GO:0016020

membrane

membrane

Details: 
Double layer of lipid molecules that encloses all cells, and, in eukaryotes, many organelles; may be a single or double lipid bilayer; also includes associated proteins.
GO Category: 
cellular_component
33
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0004386

helicase activity

helicase activity

Details: 
Catalysis of the reaction: NTP + H2O = NDP + phosphate, to drive the unwinding of a DNA or RNA helix.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0016887

ATPase activity

ATPase activity

Details: 
Catalysis of the reaction: ATP + H2O = ADP + phosphate + 2 H+. May or may not be coupled to another reaction.
GO Category: 
molecular_function
25
Total items in this category:  
GO:0043952

protein transport by the Sec complex

protein transport by the Sec complex

Details: 
The process in which unfolded proteins are transported across the cytoplasmic membrane in Gram-positive and Gram-negative bacteria by the Sec complex, in a process involving proteolytic cleavage of an N-terminal signal peptide.
GO Category: 
biological_process
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 16.87

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: