Rv1826 Glycine cleavage system H protein

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1826 gcvH Glycine cleavage system H protein CDS 2071952 2072356 + 405 134 FALSE

Rv1826 (Glycine cleavage system H protein) is predicted to be co-regulated in modules bicluster_0164 with residual 0.60 and bicluster_0356 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0164 and 0.00 and 0.00 for bicluster_0356 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Glycine cleavage system H protein glycine cleavage system protein H
Operon # Operon
1198 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608963 NP_216342.1 Run
GO:0006546

glycine catabolic process

glycine catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of glycine, aminoethanoic acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0005960

glycine cleavage complex

glycine cleavage complex

Details: 
A protein complex that catalyzes the reversible oxidation of glycine. In E. coli, it has four components: dihydrolipoamide dehydrogenase, glycine dehydrogenase (decarboxylating), lipoyl-GcvH-protein and aminomethyltransferase, also known as L, P, H, and T.
GO Category: 
cellular_component
1
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: