Rv1850 Urease alpha subunit (EC 3.5.1.5)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1850 ureC Urease alpha subunit (EC 3.5.1.5) CDS 2097961 2099694 + 1 734 577 FALSE

Rv1850 (Urease alpha subunit (EC 3.5.1.5)) is predicted to be co-regulated in modules bicluster_0248 with residual 0.48 and bicluster_0506 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4.60 and 3,700.00 for bicluster_0248 and 0.02 and 2.50 for bicluster_0506 respectively.

These modules are enriched for following go terms: nitrogen cycle metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Urease subunit alpha urease subunit alpha
Operon # Operon
1213 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Urease Atrazine degradation, Purine metabolism, Arginine and proline metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Atrazine degradation

4
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608987 NP_216366.1 Run
GO:0009039

urease activity

urease activity

Details: 
Catalysis of the reaction: urea + H2O = CO2 + 2 NH3.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0006995

cellular response to nitrogen starvation

cellular response to nitrogen starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nitrogen.
GO Category: 
biological_process
4
Total items in this category:  
GO:0009039

urease activity

urease activity

Details: 
Catalysis of the reaction: urea + H2O = CO2 + 2 NH3.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0016151

nickel cation binding

nickel cation binding

Details: 
Interacting selectively and non-covalently with nickel (Ni) cations.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0035550

urease complex

urease complex

Details: 
A multiprotein nickel-containing complex that possesses urease activity (catalysis of the hydrolysis of urea to ammonia and carbon dioxide).
GO Category: 
cellular_component
3
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.230000 0.84

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: