Rv1872c L-lactate dehydrogenase (EC 1.1.2.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1872c lldD2 L-lactate dehydrogenase (EC 1.1.2.3) CDS 2121907 2123151 - 1 245 414 FALSE

Rv1872c (L-lactate dehydrogenase (EC 1.1.2.3)) is predicted to be co-regulated in modules bicluster_0384 with residual 0.49 and bicluster_0451 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.58 for bicluster_0384 and 7,800.00 and 5,500.00 for bicluster_0451 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17997 MT1921 1087
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator TrcR
No 21 -0.09 0.959936 Primary.TSS
Mycofactocin system transcriptional regulator
No -48 0.08 0.999588 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0384
e.value: 
0.0002
Motif Bicluster: 
e.value: 
0.58
Motif Bicluster: 
0.49
bicluster_0451
e.value: 
7800
Motif Bicluster: 
e.value: 
5500
Motif Bicluster: 
0.46
Product (LegacyBRC) Product (RefSeq)
POSSIBLE L-LACTATE DEHYDROGENASE [CYTOCHROME] LLDD2 L-lactate dehydrogenase (cytochrome) LldD2
Operon # Operon
1226 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

L-lactate dehydrogenase (cytochrome) Pyruvate metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609009 NP_216388.1 Run
GO:0004460

L-lactate dehydrogenase (cytochrome) activity

L-lactate dehydrogenase (cytochrome) activity

Details: 
Catalysis of the reaction: (S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.460000 0.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: