Rv1885c Periplasmic chorismate mutase I precursor (EC 5.4.99.5)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv1885c Periplasmic chorismate mutase I precursor (EC 5.4.99.5) CDS 2134273 2134872 - 600 199 FALSE

Rv1885c (Periplasmic chorismate mutase I precursor (EC 5.4.99.5)) is predicted to be co-regulated in modules bicluster_0061 with residual 0.63 and bicluster_0066 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0061 and 47.00 and 3,300.00 for bicluster_0066 respectively.

These modules are enriched for following go terms: isomerase activity glycerol ether metabolic process, ether metabolic process, protein disulfide oxidoreductase activit..., disulfide oxidoreductase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18172 MT1933 1652
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein chorismate mutase
Operon # Operon
1234 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Chorismate mutase Phenylalanine, tyrosine and tryptophan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine, tyrosine and tryptophan biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609022 NP_216401.1 Run
GO:0004106

chorismate mutase activity

chorismate mutase activity

Details: 
Catalysis of the reaction: chorismate = prephenate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0004106

chorismate mutase activity

chorismate mutase activity

Details: 
Catalysis of the reaction: chorismate = prephenate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005737

cytoplasm

cytoplasm

Details: 
All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO Category: 
cellular_component
7
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.320000 0.71

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: