Rv1915 Isocitrate lyase (EC 4.1.3.1), group III, Mycobacterial type ICL2

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1915 aceAa Isocitrate lyase (EC 4.1.3.1), group III, Mycobacterial type ICL2 CDS 2160463 2161566 + 1 104 367 FALSE

Rv1915 (Isocitrate lyase (EC 4.1.3.1), group III, Mycobacterial type ICL2) is predicted to be co-regulated in modules bicluster_0106 with residual 0.48 and bicluster_0351 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.82 and 3.10 for bicluster_0106 and 0.00 and 0.02 for bicluster_0351 respectively.

These modules are enriched for following go terms: oxo-acid-lyase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14756 MT1966 78
Product (LegacyBRC) Product (RefSeq)
PROBABLE ISOCITRATE LYASE aceAa [FIRST PART] [ISOCITRASE] [ISOCITRATASE] [ICL] isocitrate lyase
Operon # Operon
1257 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Isocitrate lyase Glyoxylate and dicarboxylate metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609052 NP_216431.1 Run
GO:0004451

isocitrate lyase activity

isocitrate lyase activity

Details: 
Catalysis of the reaction: isocitrate = glyoxylate + succinate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0004451

isocitrate lyase activity

isocitrate lyase activity

Details: 
Catalysis of the reaction: isocitrate = glyoxylate + succinate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0006097

glyoxylate cycle

glyoxylate cycle

Details: 
A modification of the TCA cycle occurring in some plants and microorganisms, in which isocitrate is cleaved to glyoxylate and succinate. Glyoxylate can then react with acetyl-CoA to form malate.
GO Category: 
biological_process
2
Total items in this category:  
GO:0046421

methylisocitrate lyase activity

methylisocitrate lyase activity

Details: 
Catalysis of the reaction: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.120000 0.86

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: