Rv1932 Thiol peroxidase, Tpx-type (EC 1.11.1.15)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1932 tpx Thiol peroxidase, Tpx-type (EC 1.11.1.15) CDS 2183372 2183869 + 498 165 FALSE

Rv1932 (Thiol peroxidase, Tpx-type (EC 1.11.1.15)) is predicted to be co-regulated in modules bicluster_0198 with residual 0.47 and bicluster_0585 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 13.00 and 3,100.00 for bicluster_0198 and 0.06 and 350.00 for bicluster_0585 respectively.

These modules are enriched for following go terms: cofactor biosynthetic process, coenzyme metabolic process, cofactor metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15710 MT1982 1752
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0198
e.value: 
13
Motif Bicluster: 
e.value: 
3100
Motif Bicluster: 
0.47
bicluster_0585
e.value: 
0.055
Motif Bicluster: 
e.value: 
350
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Probable thiol peroxidase thiol peroxidase
Operon # Operon
1270
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Peroxiredoxin Glutathione metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609069 NP_216448.1 Run
GO:0004601

peroxidase activity

peroxidase activity

Details: 
Catalysis of the reaction: donor + hydrogen peroxide = oxidized donor + 2 H2O.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0051920

peroxiredoxin activity

peroxiredoxin activity

Details: 
Catalysis of the reaction: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
GO:0070402

NADPH binding

NADPH binding

Details: 
Interacting selectively and non-covalently with the reduced form, NADPH, of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
6
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.130000 1.94

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: