Rv1938 Monooxygenase component C / Probable epoxide hydrolase EphB (EC 3.3.2.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1938 ephB Monooxygenase component C / Probable epoxide hydrolase EphB (EC 3.3.2.9) CDS 2191027 2192097 + 1 071 356 FALSE

Rv1938 (Monooxygenase component C / Probable epoxide hydrolase EphB (EC 3.3.2.9)) is predicted to be co-regulated in modules bicluster_0336 with residual 0.51 and bicluster_0420 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.44 for bicluster_0336 and 0.00 and 0.00 for bicluster_0420 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18002 MT1988 1105
Product (LegacyBRC) Product (RefSeq)
PROBABLE EPOXIDE HYDROLASE EPHB [EPOXIDE HYDRATASE] epoxide hydrolase EphB
Operon # Operon
1272 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Microsomal epoxide hydrolase Metabolism of xenobiotics by cytochrome P450
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609075 NP_216454.1 Run
GO:0018742

epoxide hydrolase B activity

epoxide hydrolase B activity

Details: 
Catalysis of the hydrolysis of the ether in chloro- or hydroxyepoxypropane to produce chloropropane diol or glycerol. Acts on R enantiomers.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.980000 1.34

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: