Rv1981c Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1981c nrdF1 Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1) CDS 2224220 2225188 - 969 322 FALSE

Rv1981c (Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)) is predicted to be co-regulated in modules bicluster_0309 with residual 0.40 and bicluster_0519 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,400.00 and 2,000.00 for bicluster_0309 and 1,600.00 and 7,500.00 for bicluster_0519 respectively.

These modules are enriched for following go terms: organonitrogen compound metabolic proces..., carboxylic acid biosynthetic process, organic acid biosynthetic process, small molecule metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17987 MT2033 1068
Product (LegacyBRC) Product (RefSeq)
Ribonucleoside-diphosphate reductase subunit beta ribonucleotide-diphosphate reductase subunit beta
Operon # Operon
1293
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116932 YP_177853.1 Run
GO:0004748

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Details: 
Catalysis of the reaction: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin. Thioredoxin disulfide is the oxidized form of thioredoxin.
GO Category: 
molecular_function
4
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 1.07

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: