Rv2002 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase (EC 1.1.1.53)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2002 fabG3 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase (EC 1.1.1.53) CDS 2247660 2248442 + 783 260 FALSE

Rv2002 (3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase (EC 1.1.1.53)) is predicted to be co-regulated in modules bicluster_0334 with residual 0.56 and bicluster_0439 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.06 and 520.00 for bicluster_0334 and 0.00 and 0.00 for bicluster_0439 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.094 2247606 2247660
Product (LegacyBRC) Product (RefSeq)
3-alpha-[or 20-beta]-hydroxysteroid dehydrogenase 20-beta-hydroxysteroid dehydrogenase
Operon # Operon
1313
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609139 NP_216518.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008202

steroid metabolic process

steroid metabolic process

Details: 
The chemical reactions and pathways involving steroids, compounds with a 1,2,cyclopentanoperhydrophenanthrene nucleus.
GO Category: 
biological_process
1
Total items in this category:  
GO:0047044

androstan-3-alpha,17-beta-diol dehydrogenase activity

androstan-3-alpha,17-beta-diol dehydrogenase activity

Details: 
Catalysis of the reaction: NAD+ + androstan-3-alpha,17-beta-diol = 17-beta-hydroxyandrostan-3-one + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.570000 1.58

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: