Rv2006 Trehalose-6-phosphate phosphatase (EC 3.1.3.12)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2006 otsB1 Trehalose-6-phosphate phosphatase (EC 3.1.3.12) CDS 2252002 2255985 + 3 984 1 327 FALSE

Rv2006 (Trehalose-6-phosphate phosphatase (EC 3.1.3.12)) is predicted to be co-regulated in modules bicluster_0027 with residual 0.56 and bicluster_0417 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.04 for bicluster_0027 and 0.00 and 0.00 for bicluster_0417 respectively.

These modules are enriched for following go terms: disaccharide metabolic process, trehalose metabolic process, trehalose biosynthetic process, disaccharide biosynthetic process, carbohydrate biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized glycosyl hydrolase Rv2006_MT2062 trehalose-6-phosphate phosphatase OtsB1
Operon # Operon
1316
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116935 YP_177855.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0004805

trehalose-phosphatase activity

trehalose-phosphatase activity

Details: 
Catalysis of the reaction: trehalose 6-phosphate + H2O = trehalose + phosphate.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.990000 1.58

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: