Rv2043c Nicotinamidase (EC 3.5.1.19)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2043c pncA Nicotinamidase (EC 3.5.1.19) CDS 2288681 2289241 - 561 186 FALSE

Rv2043c (Nicotinamidase (EC 3.5.1.19)) is predicted to be co-regulated in modules bicluster_0125 with residual 0.48 and bicluster_0326 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 28.00 for bicluster_0125 and 0.00 and 0.40 for bicluster_0326 respectively.

These modules are enriched for following go terms: transport, establishment of localization, localization, transporter activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0125
e.value: 
0.00000000079
Motif Bicluster: 
e.value: 
28
Motif Bicluster: 
0.48
bicluster_0326
e.value: 
0.00078
Motif Bicluster: 
e.value: 
0.4
Motif Bicluster: 
0.49
Product (LegacyBRC) Product (RefSeq)
PYRAZINAMIDASE_NICOTINAMIDAS PNCA [PZase] pyrazinamidase/nicotinamidas PNCA (PZase)
Operon # Operon
1336 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Nicotinamidase Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609180 NP_216559.1 Run
GO:0008936

nicotinamidase activity

nicotinamidase activity

Details: 
Catalysis of the reaction: nicotinamide + H2O = nicotinate + NH3.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005506

iron ion binding

iron ion binding

Details: 
Interacting selectively and non-covalently with iron (Fe) ions.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0008936

nicotinamidase activity

nicotinamidase activity

Details: 
Catalysis of the reaction: nicotinamide + H2O = nicotinate + NH3.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0016811

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Details: 
Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a linear amide.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.800000 13.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: