Rv2051c Apolipoprotein N-acyltransferase (EC 2.3.1.-) in lipid-linked oligosaccharide synthesis cluster / Dolichol-phosphate mannosyltransferase (EC 2.4.1.83) in lipid-linked oligosaccharide synthesis cluster

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2051c ppm1 Apolipoprotein N-acyltransferase (EC 2.3.1.-) in lipid-linked oligosaccharide synthesis cluster /... CDS 2308131 2310755 - 2 625 874 FALSE

Rv2051c (Apolipoprotein N-acyltransferase (EC 2.3.1.-) in lipid-linked oligosaccharide synthesis cluster / Dolichol-phosphate mannosyltransferase (EC 2.4.1.83) in lipid-linked oligosaccharide synthesis cluster) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0234 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 44.00 and 9,500.00 for bicluster_0234 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.639 1596 2309159 2310920 2310755
Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15717 MT2111 1345
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0162
e.value: 
45
Motif Bicluster: 
e.value: 
5400
Motif Bicluster: 
0.54
bicluster_0234
e.value: 
44
Motif Bicluster: 
e.value: 
9500
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
Polyprenol-monophosphomannose synthase Ppm1 polyprenol-monophosphomannose synthase Ppm1
Operon # Operon
1343
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Transferring groups other than amino-acyl groups. Tyrosine metabolism, 2,4-Dichlorobenzoate degradation, Tropane, piperidine and pyridine alkaloid biosynthesis, Lipopolysaccharide biosynthesis, Diterpenoid biosynthesis, Histidine metabolism, 1,4-Dichlorobenzene degradation, Betalain biosynthesis, Anthocyanin biosynthesis, Carotenoid biosynthesis, Naphthalene and anthracene degradation, Ethylbenzene degradation, Arginine and proline metabolism, Butanoate metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Biosynthesis of type II polyketide backbone, Ether lipid metabolism, Lysine degradation, Glycosphingolipid biosynthesis - ganglio series, Fatty acid biosynthesis, Glycerophospholipid metabolism, Limonene and pinene degradation, Benzoate degradation via hydroxylation, N-Glycan biosynthesis, Methane metabolism, Tetracycline biosynthesis, Isoquinoline alkaloid biosynthesis

PATRIC

Dolichyl-phosphate beta-D-mannosyltransferase Tyrosine metabolism, 2,4-Dichlorobenzoate degradation, Tropane, piperidine and pyridine alkaloid biosynthesis, Lipopolysaccharide biosynthesis, Diterpenoid biosynthesis, Histidine metabolism, 1,4-Dichlorobenzene degradation, Betalain biosynthesis, Anthocyanin biosynthesis, Carotenoid biosynthesis, Naphthalene and anthracene degradation, Ethylbenzene degradation, Arginine and proline metabolism, Butanoate metabolism, Stilbenoid, diarylheptanoid and gingerol biosynthesis, Biosynthesis of type II polyketide backbone, Ether lipid metabolism, Lysine degradation, Glycosphingolipid biosynthesis - ganglio series, Fatty acid biosynthesis, Glycerophospholipid metabolism, Limonene and pinene degradation, Benzoate degradation via hydroxylation, N-Glycan biosynthesis, Methane metabolism, Tetracycline biosynthesis, Isoquinoline alkaloid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609188 NP_216567.1 Run
GO:0004582

dolichyl-phosphate beta-D-mannosyltransferase activity

dolichyl-phosphate beta-D-mannosyltransferase activity

Details: 
Catalysis of the reaction: GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.460000 1.47

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: