Rv2066 Cobalt-precorrin-2 C20-methyltransferase (EC / Cobalt-precorrin-3b C17-methyltransferase

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2066 cobI Cobalt-precorrin-2 C20-methyltransferase (EC / Cobalt-precorrin-3b C17-methyltransferase CDS 2323175 2324701 + 1 527 508 FALSE

Rv2066 (Cobalt-precorrin-2 C20-methyltransferase (EC / Cobalt-precorrin-3b C17-methyltransferase) is predicted to be co-regulated in modules bicluster_0117 with residual 0.46 and bicluster_0474 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 6,200.00 and 9,100.00 for bicluster_0117 and 0.24 and 18,000.00 for bicluster_0474 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18844 MT2126 3148
Product (LegacyBRC) Product (RefSeq)
Cobalamin biosynthesis protein cobIJ bifunctional S-adenosyl-L-methionine-precorrin-2 methyl transferase/precorrin-3 methylase Cob I/J
Operon # Operon
1351 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Precorrin-2 C(20)-methyltransferase Porphyrin and chlorophyll metabolism
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Porphyrin and chlorophyll metabolism

Total items in this category:  


Metabolic pathways

Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15609203 NP_216582.1 Run

plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.910000 1.62

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: