Rv2068c Beta-lactamase class A

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2068c blaC Beta-lactamase class A CDS 2325886 2326809 - 924 307 FALSE

Rv2068c (Beta-lactamase class A) is predicted to be co-regulated in modules bicluster_0061 with residual 0.63 and bicluster_0556 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0061 and 0.02 and 1.40 for bicluster_0556 respectively.

These modules are enriched for following go terms: isomerase activity cobalamin binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Beta-lactamase class A BETA-lactamase BLAC
Operon # Operon
1352 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Penicillin and cephalosporin biosynthesis

2
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609205 NP_216584.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008800

beta-lactamase activity

beta-lactamase activity

Details: 
Catalysis of the reaction: a beta-lactam + H2O = a substituted beta-amino acid.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0030655

beta-lactam antibiotic catabolic process

beta-lactam antibiotic catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of a beta-lactam antibiotic, any member of a class of natural or semisynthetic antibiotics whose characteristic feature is a strained, four-membered beta-lactam ring. They include the penicillins and many of the cephalosporins.
GO Category: 
biological_process
1
Total items in this category:  
GO:0033250

penicillinase activity

penicillinase activity

Details: 
Catalysis of the reaction: a penicillin + H2O = a substituted beta-amino acid derivative of the penicillin.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0033251

cephalosporinase activity

cephalosporinase activity

Details: 
Catalysis of the reaction: a cephalosporin + H2O = a substituted beta-amino acid derivative of the cephalosporin.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0046677

response to antibiotic

response to antibiotic

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
GO Category: 
biological_process
15
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.510000 0.96

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: