Rv2073c Oxidoreductase, short-chain dehydrogenase/reductase family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2073c Oxidoreductase, short-chain dehydrogenase/reductase family CDS 2330214 2330963 - 750 249 FALSE

Rv2073c (Oxidoreductase, short-chain dehydrogenase/reductase family) is predicted to be co-regulated in modules bicluster_0057 with residual 0.63 and bicluster_0497 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.02 for bicluster_0057 and 310.00 and 800.00 for bicluster_0497 respectively.

These modules are enriched for following go terms: tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce... 3-hydroxyacyl-CoA dehydrogenase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized oxidoreductase Rv2073c_MT2133 shortchain dehydrogenase
Operon # Operon
1355
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609210 NP_216589.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.890000 12.49

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: