Rv2088 Serine/threonine protein kinase PknJ (EC 2.7.11.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2088 pknJ Serine/threonine protein kinase PknJ (EC 2.7.11.1) CDS 2344411 2346180 + 1 770 589 FALSE

Rv2088 (Serine/threonine protein kinase PknJ (EC 2.7.11.1)) is predicted to be co-regulated in modules bicluster_0103 with residual 0.34 and bicluster_0529 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 470.00 and 970.00 for bicluster_0103 and 0.00 and 0.08 for bicluster_0529 respectively.

These modules are enriched for following go terms: formyltetrahydrofolate deformylase activ....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14741 MT2149 548
Product (LegacyBRC) Product (RefSeq)
Probable serine_threonine-protein kinase pknJ transmembrane serine/threonine-protein kinase J
Operon # Operon
1366
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609225 NP_216604.1 Run
GO:0004674

protein serine/threonine kinase activity

protein serine/threonine kinase activity

Details: 
Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.800000 2.02

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: