Rv2090 DNA polymerase I (EC 2.7.7.7)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2090 DNA polymerase I (EC 2.7.7.7) CDS 2347373 2348554 + 1 182 393 FALSE

Rv2090 (DNA polymerase I (EC 2.7.7.7)) is predicted to be co-regulated in modules bicluster_0122 with residual 0.57 and bicluster_0133 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.10 and 6.50 for bicluster_0122 and 0.58 and 32.00 for bicluster_0133 respectively.

These modules are enriched for following go terms: cellular protein modification process, protein modification process, phosphorylation, protein kinase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
5&apos-3&apos exonuclease 5'-3' exonuclease
Operon # Operon
1368
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

DNA-directed DNA polymerase Purine metabolism, Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609227 NP_216606.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.100000 26.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: