Rv2112c Pup ligase PafA' paralog, possible component of postulated heterodimer PafA-PafA'

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2112c dop Pup ligase PafA' paralog, possible component of postulated heterodimer PafA-PafA' CDS 2370905 2372569 - 1 665 554 FALSE

Rv2112c (Pup ligase PafA' paralog, possible component of postulated heterodimer PafA-PafA') is predicted to be co-regulated in modules bicluster_0236 with residual 0.53 and bicluster_0436 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 1,800.00 for bicluster_0236 and 140.00 and 9,000.00 for bicluster_0436 respectively.

These modules are enriched for following go terms: cellular macromolecule catabolic process, tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14874 MT2172 64
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
1382
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609249 NP_216628.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0016811

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Details: 
Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a linear amide.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0070490

protein pupylation

protein pupylation

Details: 
The process in which a Pup protein is conjugated to a target protein via an isopeptide bond between the carboxyl terminus of Pup and the epsilon-amino group of a lysine residue of the target protein.
GO Category: 
biological_process
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.860000 1.54

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: