Rv2118c RNA methyltransferase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2118c RNA methyltransferase CDS 2377470 2378312 - 843 280 FALSE

Rv2118c (RNA methyltransferase) is predicted to be co-regulated in modules bicluster_0217 with residual 0.47 and bicluster_0273 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.09 for bicluster_0217 and 0.00 and 1.70 for bicluster_0273 respectively.

These modules are enriched for following go terms: vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce..., cofactor metabolic process, carboxylic acid biosynthetic process, organic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE RNA METHYLTRANSFERASE RNA methyltransferase
Operon # Operon
1386
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609255 NP_216634.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008757

S-adenosylmethionine-dependent methyltransferase activity

S-adenosylmethionine-dependent methyltransferase activity

Details: 
Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to a substrate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0016429

tRNA (adenine-N1-)-methyltransferase activity

tRNA (adenine-N1-)-methyltransferase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N1-methyladenine.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0030488

tRNA methylation

tRNA methylation

Details: 
The posttranscriptional addition of methyl groups to specific residues in a tRNA molecule.
GO Category: 
biological_process
1
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.32

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: