Rv2174 Carotene biosynthesis associated membrane protein

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2174 mptA Carotene biosynthesis associated membrane protein CDS 2435909 2437459 + 1 551 516 FALSE

Rv2174 (Carotene biosynthesis associated membrane protein) is predicted to be co-regulated in modules bicluster_0123 with residual 0.55 and bicluster_0174 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 4,200.00 for bicluster_0123 and 3,200.00 and 10,000.00 for bicluster_0174 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, fatty acid biosynthetic process, carboxylic acid metabolic process, fatty acid synthase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.733 831 2436740 2435942 2435909
Product (LegacyBRC) Product (RefSeq)
Possible conserved integral membrane protein integral membrane protein
Operon # Operon
1422 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609311 NP_216690.1 Run
GO:0000030

mannosyltransferase activity

mannosyltransferase activity

Details: 
Catalysis of the transfer of a mannosyl group to an acceptor molecule, typically another carbohydrate or a lipid.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: