Rv2181 Possible membrane protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2181 Possible membrane protein CDS 2443302 2444585 + 1 284 427 FALSE

Rv2181 (Possible membrane protein) is predicted to be co-regulated in modules bicluster_0192 with residual 0.54 and bicluster_0281 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4,500.00 and 12,000.00 for bicluster_0192 and 140.00 and 1,200.00 for bicluster_0281 respectively.

These modules are enriched for following go terms: cellular macromolecular complex assembly, cellular protein complex assembly, protein complex assembly, macromolecular complex assembly, protein complex biogenesis acetate-CoA ligase activity.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17573 MT2236 1592
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -133 -0.19 0.133859 CDS
Motif 1 Motif 2 Residual
bicluster_0192
e.value: 
4500
Motif Bicluster: 
e.value: 
12000
Motif Bicluster: 
0.54
bicluster_0281
e.value: 
140
Motif Bicluster: 
e.value: 
1200
Motif Bicluster: 
0.48
Product (LegacyBRC) Product (RefSeq)
Probable conserved integral membrane protein [Putative uncharacterized protein] integral membrane protein
Operon # Operon
1428 Rv2181
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609318 NP_216697.1 Run
GO:0000026

alpha-1,2-mannosyltransferase activity

alpha-1,2-mannosyltransferase activity

Details: 
Catalysis of the transfer of a mannose residue to an oligosaccharide, forming an alpha-(1->2) linkage.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.110000 0.88

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.