Rv2197c Possible membrane protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2197c Possible membrane protein CDS 2461504 2462148 - 645 214 FALSE

Rv2197c (Possible membrane protein) is predicted to be co-regulated in modules bicluster_0252 with residual 0.55 and bicluster_0564 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 930.00 and 3,100.00 for bicluster_0252 and 0.55 and 7.40 for bicluster_0564 respectively.

These modules are enriched for following go terms: regulation of cell shape, regulation of anatomical structure morph..., regulation of cell morphogenesis, regulation of developmental process, cell morphogenesis, anatomical structure morphogenesis, developmental process, cellular component morphogenesis, single-organism developmental process, anatomical structure development, cellular developmental process, regulation of cellular component organiz..., cellular component organization .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18400 MT2253 2195
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 5 of 5
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -144 -0.18 0.999609 CDS
DNA-binding response regulator TrcR
No 24 -0.07 0.875965 CDS
No -98 -0.17 0.406567 CDS
Transcriptional regulator, TetR family
No 39 0.13 0.704948 CDS
Two component DNA binding Transcriptional regulatory protein TcrA
No 28 -0.11 0.682317 CDS
Motif 1 Motif 2 Residual
bicluster_0252
e.value: 
930
Motif Bicluster: 
e.value: 
3100
Motif Bicluster: 
0.55
bicluster_0564
e.value: 
0.55
Motif Bicluster: 
e.value: 
7.4
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv2197c_MT2253 transmembrane protein
Operon # Operon
1441 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609334 NP_216713.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.930000 1.08

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: