Rv2204c probable iron binding protein from the HesB_IscA_SufA family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2204c probable iron binding protein from the HesB_IscA_SufA family CDS 2468931 2469287 - 357 118 FALSE

Rv2204c (probable iron binding protein from the HesB_IscA_SufA family) is predicted to be co-regulated in modules bicluster_0322 with residual 0.56 and bicluster_0389 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 350.00 for bicluster_0322 and 0.00 and 0.02 for bicluster_0389 respectively.

These modules are enriched for following go terms: mitochondrial electron transport, NADH t..., mitochondrial ATP synthesis coupled elec....

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
RNA polymerase sigma-70 factor
No -35 0.11 0.855718 Primary.TSS
RNA polymerase sigma-54 factor RpoN
No -25 0.46 0.0101722 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0322
e.value: 
0.0000039
Motif Bicluster: 
e.value: 
350
Motif Bicluster: 
0.56
bicluster_0389
e.value: 
0.003
Motif Bicluster: 
e.value: 
0.017
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Rv2204c
Operon # Operon
1446
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609341 NP_216720.1 Run
GO:0003674

molecular_function

molecular_function

Details: 
Elemental activities, such as catalysis or binding, describing the actions of a gene product at the molecular level. A given gene product may exhibit one or more molecular functions.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: