Rv2208 Cobalamin synthase

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2208 cobS Cobalamin synthase CDS 2472493 2473242 + 750 249 FALSE

Rv2208 (Cobalamin synthase) is predicted to be co-regulated in modules bicluster_0034 with residual 0.54 and bicluster_0146 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 57.00 and 260.00 for bicluster_0034 and 1,200.00 and 3,200.00 for bicluster_0146 respectively.

These modules are enriched for following go terms: phosphotransferase activity, for other s... vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Cobalamin synthase cobalamin synthase
Operon # Operon
1449 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Porphyrin and chlorophyll metabolism

Total items in this category:  


Metabolic pathways

Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15609345 NP_216724.1 Run

cobalamin 5'-phosphate synthase activity

cobalamin 5'-phosphate synthase activity

Catalysis of the reaction: adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate = adenosylcobalamin-5'-phosphate + GMP.
GO Category: 
Total items in this category:  

cobalamin biosynthetic process

cobalamin biosynthetic process

The chemical reactions and pathways resulting in the formation of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.350000 0.76

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: