Rv2212 Adenylate cyclase (EC 4.6.1.1)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2212 Adenylate cyclase (EC 4.6.1.1) CDS 2477190 2478326 + 1 137 378 FALSE

Rv2212 (Adenylate cyclase (EC 4.6.1.1)) is predicted to be co-regulated in modules bicluster_0023 with residual 0.49 and bicluster_0038 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 200.00 and 300.00 for bicluster_0023 and 5,200.00 and 7,700.00 for bicluster_0038 respectively.

These modules are enriched for following go terms: adenylyltransferase activity, transferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.038 15 2477205 -3 2477202 2477190
Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14919 MT2268 248
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv2212_MT2268
Operon # Operon
1453 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Adenylate cyclase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609349 NP_216728.1 Run
GO:0005504

fatty acid binding

fatty acid binding

Details: 
Interacting selectively and non-covalently with fatty acids, aliphatic monocarboxylic acids liberated from naturally occurring fats and oils by hydrolysis.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.530000 1.34

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: