Rv2214c Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2214c ephD Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-) CDS 2479923 2481701 - 1 779 592 FALSE

Rv2214c (Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-)) is predicted to be co-regulated in modules bicluster_0019 with residual 0.52 and bicluster_0574 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 27.00 and 3,700.00 for bicluster_0019 and 26.00 and 2,900.00 for bicluster_0574 respectively.

These modules are enriched for following go terms: fatty acid biosynthetic process, monocarboxylic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18167 MT2270 3244
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
Repressed -10 -1.93 4.2039e-45 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0019
e.value: 
27
Motif Bicluster: 
e.value: 
3700
Motif Bicluster: 
0.52
bicluster_0574
e.value: 
26
Motif Bicluster: 
e.value: 
2900
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
Probable oxidoreductase ephD short chain dehydrogenase
Operon # Operon
1454
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609351 NP_216730.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.450000 1.02

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: