Rv2215 Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2215 dlaT Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex... CDS 2481965 2483626 + 1 662 553 FALSE

Rv2215 (Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)) is predicted to be co-regulated in modules bicluster_0019 with residual 0.52 and bicluster_0574 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 27.00 and 3,700.00 for bicluster_0019 and 26.00 and 2,900.00 for bicluster_0574 respectively.

These modules are enriched for following go terms: fatty acid biosynthetic process, monocarboxylic acid biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex dihydrolipoamide acetyltransferase
Operon # Operon
1455 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609352 NP_216731.1 Run
GO:0004149

dihydrolipoyllysine-residue succinyltransferase activity

dihydrolipoyllysine-residue succinyltransferase activity

Details: 
Catalysis of the reaction: succinyl-CoA + dihydrolipoamide = CoA + S-succinyldihydrolipoamide.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0031405

lipoic acid binding

lipoic acid binding

Details: 
Interacting selectively and non-covalently with lipoic acid, 1,2-dithiolane-3-pentanoic acid.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0045254

pyruvate dehydrogenase complex

pyruvate dehydrogenase complex

Details: 
Complex that carries out the oxidative decarboxylation of pyruvate to form acetyl-CoA; comprises subunits possessing three catalytic activities: pyruvate dehydrogenase (E1), dihydrolipoamide S-acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3).
GO Category: 
cellular_component
4
Total items in this category:  
GO:0045454

cell redox homeostasis

cell redox homeostasis

Details: 
Any process that maintains the redox environment of a cell or compartment within a cell.
GO Category: 
biological_process
9
Total items in this category:  
GO:0004591

oxoglutarate dehydrogenase (succinyl-transferring) activity

oxoglutarate dehydrogenase (succinyl-transferring) activity

Details: 
Catalysis of the reaction: 2-oxoglutarate + lipoamide = S-succinyldihydrolipoamide + CO2.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: