Rv2220 Glutamine synthetase type I (EC 6.3.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2220 glnA1 Glutamine synthetase type I (EC 6.3.1.2) CDS 2487615 2489051 + 1 437 478 FALSE

Rv2220 (Glutamine synthetase type I (EC 6.3.1.2)) is predicted to be co-regulated in modules bicluster_0416 with residual 0.55 and bicluster_0586 with residual 0.63.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.34 and 65.00 for bicluster_0416 and 1,800.00 and 5,500.00 for bicluster_0586 respectively.

These modules are enriched for following go terms: nucleic acid metabolic process, heterocyclic compound binding, organic cyclic compound binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Possible transcriptional regulatory protein
No -88 -0.16 0.661235 Primary.TSS
Transcriptional regulator, PadR family
No 50 0.08 0.975071 Primary.TSS
Transcriptional regulator, TetR family
No 64 0.03 0.973121 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0416
e.value: 
0.34
Motif Bicluster: 
e.value: 
65
Motif Bicluster: 
0.55
bicluster_0586
e.value: 
1800
Motif Bicluster: 
e.value: 
5500
Motif Bicluster: 
0.63
Product (LegacyBRC) Product (RefSeq)
Rv2220 glutamine synthetase GLNA1 (glutamine synthase) (GS-I)
Operon # Operon
1458
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Nitrogen metabolism

24
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609357 NP_216736.1 Run
GO:0004356

glutamate-ammonia ligase activity

glutamate-ammonia ligase activity

Details: 
Catalysis of the reaction: L-glutamate + ATP + NH(4)(+) = L-glutamine + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004356

glutamate-ammonia ligase activity

glutamate-ammonia ligase activity

Details: 
Catalysis of the reaction: L-glutamate + ATP + NH(4)(+) = L-glutamine + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006542

glutamine biosynthetic process

glutamine biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glutamine, 2-amino-4-carbamoylbutanoic acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: